MRPL49 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPL49 transfected lysate.
Western Blot
Lane 1: MRPL49 transfected lysate ( 18.37 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL49 full-length
Host
Human
Theoretical MW (kDa)
18.37
Interspecies Antigen Sequence
Mouse (83); Rat (84)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL49 antibody (H00000740-B01) by Western Blots.
SDS-PAGE Gel
MRPL49 transfected lysate.
Western Blot
Lane 1: MRPL49 transfected lysate ( 18.37 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL49
Entrez GeneID
740GeneBank Accession#
NM_004927.2Protein Accession#
NP_004918.1Gene Name
MRPL49
Gene Alias
C11orf4, L49mt, MGC10656, NOF, NOF1
Gene Description
mitochondrial ribosomal protein L49
Omim ID
606866Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene and the gene for the HRD1 protein use in their respective 3' UTRs some of the same genomic sequence. Pseudogenes corresponding to this gene are found on chromosomes 5q and 8p. [provided by RefSeq
Other Designations
neighbor of FAU|next to FAU
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