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BYSL 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000705-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-BYSL full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 48.18
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BYSL antibody (H00000705-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000705-T02
    BYSL transfected lysate.
    Western Blot
    QC Testing of H00000705-T02
    Lane 1: BYSL transfected lysate ( 48.18 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 705
  • Gene Name:
  • BYSL
  • Gene Alias:
  • BYSTIN
  • Gene Description:
  • bystin-like
  • Gene Summary:
  • Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000039776,by the ribosomal protein s6 gene, drosophila, homolog-like,bystin
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