BYSL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BYSL transfected lysate
Western Blot
Lane 1: BYSL transfected lysate ( 35.75 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BYSL full-length
Host
Human
Theoretical MW (kDa)
35.75
Interspecies Antigen Sequence
Mouse (94); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BYSL antibody (H00000705-B01) by Western Blots.
SDS-PAGE Gel
BYSL transfected lysate
Western Blot
Lane 1: BYSL transfected lysate ( 35.75 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BYSL
Entrez GeneID
705GeneBank Accession#
BC007340Protein Accession#
AAH07340Gene Name
BYSL
Gene Alias
BYSTIN
Gene Description
bystin-like
Omim ID
603871Gene Ontology
HyperlinkGene Summary
Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin, and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin, tastin, and the cytokeratins, suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10, the levels of trophinin, tastin, and bystin decreased and then disappeared from placental villi. [provided by RefSeq
Other Designations
OTTHUMP00000039776|by the ribosomal protein s6 gene, drosophila, homolog-like|bystin
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Interactome
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