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Last updated: 2016/12/11
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BTF3 monoclonal antibody (M01), clone 3C4-2E11

  • Catalog # : H00000689-M01
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full length recombinant BTF3.
  • Immunogen:
  • BTF3 (AAH08062, 1 a.a. ~ 162 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG1 kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00000689-M01
    Western Blot detection against Immunogen (43.56 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of BTF3 expression in transfected 293T cell line by BTF3 monoclonal antibody (M01), clone 3C4-2E11.

    Lane 1: BTF3 transfected lysate(17.7 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged BTF3 is approximately 0.03ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 689
  • Gene Name:
  • BTF3
  • Gene Alias:
  • BETA-NAC,BTF3a,BTF3b,NACB
  • Gene Description:
  • basic transcription factor 3
  • Gene Summary:
  • This gene encodes the basic transcription factor 3. This protein forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000128197,RNA polymerase B transcription factor 3,nascent-polypeptide-associated complex beta polypeptide
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