BTD 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BTD full-length
Host
Human
Theoretical MW (kDa)
59.84
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BTD antibody (H00000686-B01) by Western Blots.
SDS-PAGE Gel
BTD transfected lysate.
Western Blot
Lane 1: BTD transfected lysate ( 59.84 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BTD
Entrez GeneID
686GeneBank Accession#
NM_000060.2Protein Accession#
NP_000051.1Gene Name
BTD
Gene Alias
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Gene Description
biotinidase
Gene Ontology
HyperlinkGene Summary
Biotinidase functions to recycle biotin in the body by cleaving biocytin (biotin-epsilon-lysine), a normal product of carboxylase degradation, resulting in regeneration of free biotin. Biotinidase has also been shown to have biotinyl-transferase activity. Defects in the biotinidase gene cause multiple carboxylase deficiency. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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