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Last updated: 2016/12/4
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BRAF (Human) Recombinant Protein (Q01)

  • Catalog # : H00000673-Q01
  • Visit Frequency :
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  • Specification
  • Product Description:
  • Human BRAF partial ORF ( NP_004324, 346 a.a. - 445 a.a.) recombinant protein with GST-tag at N-terminal.
  • Sequence:
  • FRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRD
  • Host:
  • Wheat Germ (in vitro)
  • Theoretical MW (kDa):
  • 36.74
  • Purification:
  • Glutathione Sepharose 4 Fast Flow
  • Quality Control Testing:
  • 12.5% SDS-PAGE Stained with Coomassie Blue.

    QC Testing of H00000673-Q01
  • Storage Buffer:
  • 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Note:
  • Best use within three months from the date of receipt of this protein.
  • Applications
  • Enzyme-linked Immunoabsorbent Assay
  • Western Blot (Recombinant protein)
  • Antibody Production
  • Protein Array
  • Application Image
  • Enzyme-linked Immunoabsorbent Assay
  • Western Blot (Recombinant protein)
  • Antibody Production
  • Protein Array
  • Gene Information
  • Entrez GeneID:
  • 673
  • Gene Name:
  • BRAF
  • Gene Alias:
  • B-RAF1,BRAF1,FLJ95109,MGC126806,MGC138284,RAFB1
  • Gene Description:
  • v-raf murine sarcoma viral oncogene homolog B1
  • Gene Summary:
  • This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq
  • Other Designations:
  • 94 kDa B-raf protein,B-Raf proto-oncogene serine/threonine-protein kinase (p94),Murine sarcoma viral (v-raf) oncogene homolog B1
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