POLR3D 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
POLR3D transfected lysate.
Western Blot
Lane 1: POLR3D transfected lysate ( 44.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-POLR3D full-length
Host
Human
Theoretical MW (kDa)
44.4
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-POLR3D antibody (H00000661-B01P) by Western Blots.
SDS-PAGE Gel
POLR3D transfected lysate.
Western Blot
Lane 1: POLR3D transfected lysate ( 44.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — POLR3D
Entrez GeneID
661GeneBank Accession#
NM_001722.2Protein Accession#
NP_001713.2Gene Name
POLR3D
Gene Alias
BN51T, RPC4, RPC53, TSBN51
Gene Description
polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
Omim ID
187280Gene Ontology
HyperlinkGene Summary
This gene complements a temperature-sensitive mutant isolated from the BHK-21 Syrian hamster cell line. It leads to a block in progression through the G1 phase of the cell cycle at nonpermissive temperatures. [provided by RefSeq
Other Designations
BN51 (BHK21) temperature sensitivity complementing|polymerase (RNA) III (DNA directed) polypeptide D|temperature sensitive complementation, cell cycle specific, tsBN51
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Interactome
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Pathway
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