BMP2 monoclonal antibody (M03), clone 4B12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant BMP2.
Immunogen
BMP2 (NP_001191, 283 a.a. ~ 396 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BMP2 is 1 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TGFB2 and BMP2. HeLa cells were stained with anti-TGFB2 rabbit purified polyclonal 1:1200 and anti-BMP2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — BMP2
Entrez GeneID
650GeneBank Accession#
NM_001200Protein Accession#
NP_001191Gene Name
BMP2
Gene Alias
BMP2A
Gene Description
bone morphogenetic protein 2
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq
Other Designations
OTTHUMP00000030228
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Interactome
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Pathway
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Disease
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Publication Reference
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Antibody-mediated osseous regeneration: a novel strategy for bioengineering bone by immobilized anti-bone morphogenetic protein-2 antibodies.
Freire MO, You HK, Kook JK, Choi JH, Zadeh HH.
Tissue Engineering. Part A 2011 Dec; 17(23-24):2911.
Application:Func, Rat, Calvaria.
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Antibody-mediated osseous regeneration: a novel strategy for bioengineering bone by immobilized anti-bone morphogenetic protein-2 antibodies.
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