BCL9 monoclonal antibody (M01), clone 2D4
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant BCL9.
Immunogen
BCL9 (NP_004317, 1036 a.a. ~ 1135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DDDSPPARSPNLPSMNNMPGMGINTQNPRISGPNPVVPMPTLSPMGMTQPLSHSNQMPSPNAVGPNIPPHGVPMGPGLMSHNPIMGHGSQEPPMVPQGRM
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BCL9 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to BCL9 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — BCL9
Entrez GeneID
607GeneBank Accession#
NM_004326Protein Accession#
NP_004317Gene Name
BCL9
Gene Alias
LGS, MGC131591
Gene Description
B-cell CLL/lymphoma 9
Omim ID
602597Gene Ontology
HyperlinkGene Summary
BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies. [provided by RefSeq
Other Designations
OTTHUMP00000015909
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Interactome
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Disease
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Publication Reference
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A novel β-catenin/BCL9 complex inhibitor blocks oncogenic Wnt signaling and disrupts cholesterol homeostasis in colorectal cancer.
Helen Tanton, Tomasz Sewastianik, Hyuk-Soo Seo, David Remillard, Roodolph St Pierre, Pratyusha Bala, Daulet Aitymbayev, Peter Dennis, Keith Adler, Ezekiel Geffken, Zoe Yeoh, Nicholas Vangos, Filip Garbicz, David Scott, Nilay Sethi, James Bradner, Sirano Dhe-Paganon, Ruben D Carrasco.
Science Advances 2022 Apr; 8(17):eabm3108.
Application:WB, Human, Colo320, HCT116 cells.
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BCL9 provides multi-cellular communication properties in colorectal cancer by interacting with paraspeckle proteins.
Jiang M, Kang Y, Sewastianik T, Wang J, Tanton H, Alder K, Dennis P, Xin Y, Wang Z, Liu R, Zhang M, Huang Y, Loda M, Srivastava A, Chen R, Liu M, Carrasco RD.
Nature Communications 2020 Jan; 11(1):19.
Application:IP, WB-Ce, WB-Tr, Human, Colo320, DLD-1, LS174T, RKO, SW620 cells.
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A novel β-catenin/BCL9 complex inhibitor blocks oncogenic Wnt signaling and disrupts cholesterol homeostasis in colorectal cancer.
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