BCAT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BCAT1 full-length
Host
Human
Theoretical MW (kDa)
35.31
Interspecies Antigen Sequence
Mouse (84); Rat (74)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BCAT1 antibody (H00000586-B01) by Western Blots.
SDS-PAGE Gel
BCAT1 transfected lysate.
Western Blot
Lane 1: BCAT1 transfected lysate ( 35.31 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BCAT1
Entrez GeneID
586GeneBank Accession#
BC033864Protein Accession#
AAH33864Gene Name
BCAT1
Gene Alias
BCT1, DKFZp686E12175, ECA39, MECA39, PNAS-121, PP18
Gene Description
branched chain aminotransferase 1, cytosolic
Omim ID
113520Gene Ontology
HyperlinkGene Summary
This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. [provided by RefSeq
Other Designations
branched-chain-amino-acid aminotransferase, cytosolic|placental protein 18
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Interactome
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