BCAT1 monoclonal antibody (M02), clone 1F8
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More Files
- More Functions
Western Blot (Tissue lysate)
BCAT1 monoclonal antibody (M02), clone 1F8. Western Blot analysis of BCAT1 expression in human kidney.
Western Blot (Cell lysate)
BCAT1 monoclonal antibody (M02), clone 1F8. Western Blot analysis of BCAT1 expression in MCF-7.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BCAT1 is 1 ng/ml as a capture antibody.
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant BCAT1.
Immunogen
BCAT1 (AAH33864, 1 a.a. ~ 320 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (74)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
BCAT1 monoclonal antibody (M02), clone 1F8. Western Blot analysis of BCAT1 expression in human kidney.Western Blot (Cell lysate)
BCAT1 monoclonal antibody (M02), clone 1F8. Western Blot analysis of BCAT1 expression in MCF-7.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BCAT1 is 1 ng/ml as a capture antibody.ELISA
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Gene Info — BCAT1
Entrez GeneID
586GeneBank Accession#
BC033864Protein Accession#
AAH33864Gene Name
BCAT1
Gene Alias
BCT1, DKFZp686E12175, ECA39, MECA39, PNAS-121, PP18
Gene Description
branched chain aminotransferase 1, cytosolic
Omim ID
113520Gene Ontology
HyperlinkGene Summary
This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. [provided by RefSeq
Other Designations
branched-chain-amino-acid aminotransferase, cytosolic|placental protein 18
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Interactome
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Pathway
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Disease
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Publication Reference
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LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis.
Ji D, Jiang C, Zhang L, Liang N, Jiang T, Yang B, Liang H.
Journal of Cellular Physiology 2018 Sep; [Epub].
Application：WB, Human, HepG2 cells.
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LncRNA CRNDE promotes hepatocellular carcinoma cell proliferation, invasion, and migration through regulating miR-203/ BCAT1 axis.
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