BBS4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
BBS4 transfected lysate.
Western Blot
Lane 1: BBS4 transfected lysate ( 57.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BBS4 full-length
Host
Human
Theoretical MW (kDa)
57.2
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BBS4 antibody (H00000585-B01) by Western Blots.
SDS-PAGE Gel
BBS4 transfected lysate.
Western Blot
Lane 1: BBS4 transfected lysate ( 57.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BBS4
Entrez GeneID
585GeneBank Accession#
NM_033028.2Protein Accession#
-Gene Name
BBS4
Gene Alias
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Gene Description
Bardet-Biedl syndrome 4
Gene Ontology
HyperlinkGene Summary
This gene is a member of the Bardet-Biedl syndrome (BBS) gene family. Bardet-Biedl syndrome is an autosomal recessive disorder characterized by severe pigmentary retinopathy, obesity, polydactyly, renal malformation and mental retardation. The proteins encoded by BBS gene family members are structurally diverse. The similar phenotypes exhibited by mutations in BBS gene family members are likely due to the protein's shared roles in cilia formation and function. Many BBS proteins localize to the basal bodies, ciliary axonemes, and pericentriolar regions of cells. BBS proteins may also be involved in intracellular trafficking via microtubule-related transport. The protein encoded by this gene has sequence similarity to O-linked N-acetylglucosamine (O-GlcNAc) transferases in plants and archaebacteria and in human forms a multi-protein "BBSome" complex with six other BBS proteins. Alternative splice variants have been described but their predicted protein products have not been experimentally verified
Other Designations
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Interactome
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Disease
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