B2M monoclonal antibody (M01J), clone 3F9-2C2
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More Files
- More Functions
Western Blot (Cell lysate)
B2M monoclonal antibody (M01J), clone 3F9-2C2. Western Blot analysis of B2M expression in U-2 OS.
Western Blot (Transfected lysate)
Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01J), clone 3F9-2C2.
Lane 1: B2M transfected lysate (Predicted MW: 13.7 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged B2M is 1 ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi ( Cat # H00000567-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody (M01), clone 3F9-2C2 (Cat # H00000567-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CALR and B2M. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-B2M mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to B2M on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (38.83 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant B2M.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
B2M (AAH32589.1, 1 a.a. ~ 119 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSRSVALAVLALLSLSGLEAIQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.83 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
B2M monoclonal antibody (M01J), clone 3F9-2C2. Western Blot analysis of B2M expression in U-2 OS.Western Blot (Transfected lysate)
Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01J), clone 3F9-2C2.
Lane 1: B2M transfected lysate (Predicted MW: 13.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged B2M is 1 ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi ( Cat # H00000567-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody (M01), clone 3F9-2C2 (Cat # H00000567-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between CALR and B2M. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-B2M mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to B2M on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — B2M
Entrez GeneID
567GeneBank Accession#
BC032589.1Protein Accession#
AAH32589.1Gene Name
B2M
Gene Alias
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Gene Description
beta-2-microglobulin
Gene Ontology
HyperlinkGene Summary
This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia
Other Designations
beta chain of MHC class I molecules|beta-2-microglobin
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Interactome
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Pathway
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Disease
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