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ATP6V0B 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000533-T04
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ATP6V0B full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 21.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ATP6V0B antibody (H00000533-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000533-T04
    ATP6V0B transfected lysate.
    Western Blot
    QC Testing of H00000533-T04
    Lane 1: ATP6V0B transfected lysate ( 21.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 533
  • Gene Name:
  • ATP6V0B
  • Gene Alias:
  • Gene Description:
  • ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
  • Gene Summary:
  • This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is part of the transmembrane V0 domain and is the human counterpart of yeast VMA16. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq
  • Other Designations:
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 21kD,ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c'',H(+)-transporting two-sector ATPase, subunit F,OTTHUMP00000010012,V-ATPase subunit c'',vacuolar ATP synthase 21 kDa proteolipid subunit
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