ATP6V1E1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ATP6V1E1 transfected lysate.
Western Blot
Lane 1: ATP6V1E1 transfected lysate ( 26.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP6V1E1 full-length
Host
Human
Theoretical MW (kDa)
26.1
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP6V1E1 antibody (H00000529-D01P) by Western Blots.
SDS-PAGE Gel
ATP6V1E1 transfected lysate.
Western Blot
Lane 1: ATP6V1E1 transfected lysate ( 26.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP6V1E1
Entrez GeneID
529GeneBank Accession#
NM_001696.3Protein Accession#
NP_001687.1Gene Name
ATP6V1E1
Gene Alias
ATP6E, ATP6E2, ATP6V1E, P31, Vma4
Gene Description
ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
Omim ID
108746Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD|H(+)-transporting two-sector ATPase, 31kDa subunit|H+-transporting ATP synthase chain E, vacuolar|V-ATPase, subunit E|vacuolar H+ ATPase E1
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Interactome
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