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ATP6V1E1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000529-T04
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ATP6V1E1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 26.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ATP6V1E1 antibody (H00000529-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000529-T04
    ATP6V1E1 transfected lysate.
    Western Blot
    QC Testing of H00000529-T04
    Lane 1: ATP6V1E1 transfected lysate ( 26.10 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 529
  • Gene Name:
  • ATP6V1E1
  • Gene Alias:
  • ATP6E,ATP6E2,ATP6V1E,P31,Vma4
  • Gene Description:
  • ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E1
  • Gene Summary:
  • This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain E subunit isoforms. Pseudogenes for this gene have been found in the genome. [provided by RefSeq
  • Other Designations:
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 31kD,H(+)-transporting two-sector ATPase, 31kDa subunit,H+-transporting ATP synthase chain E, vacuolar,V-ATPase, subunit E,vacuolar H+ ATPase E1
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