ATP6V1B2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP6V1B2 full-length
Host
Human
Theoretical MW (kDa)
56.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP6V1B2 antibody (H00000526-B01P) by Western Blots.
SDS-PAGE Gel
ATP6V1B2 transfected lysate.
Western Blot
Lane 1: ATP6V1B2 transfected lysate ( 56.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP6V1B2
Entrez GeneID
526GeneBank Accession#
BC030640Protein Accession#
AAH30640.1Gene Name
ATP6V1B2
Gene Alias
ATP6B1B2, ATP6B2, HO57, VATB, VPP3, Vma2
Gene Description
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Omim ID
606939Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2|ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2|H+ transporting two-sector ATPase|V-ATPase B2 subunit|endomembrane proton pump 58 kDa subu
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Interactome
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Disease
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