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ATP6V1B1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000525-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ATP6V1B1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 56.8
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ATP6V1B1 antibody (H00000525-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000525-T02
    ATP6V1B1 transfected lysate.
    Western Blot
    QC Testing of H00000525-T02
    Lane 1: ATP6V1B1 transfected lysate ( 56.80 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 525
  • Gene Name:
  • ATP6V1B1
  • Gene Alias:
  • ATP6B1,MGC32642,RTA1B,VATB,VMA2,VPP3
  • Gene Description:
  • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
  • Gene Summary:
  • This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. [provided by RefSeq
  • Other Designations:
  • H(+)-transporting two-sector ATPase, 58kD subunit,H+-ATPase beta 1 subunit,V-ATPase B1 subunit,endomembrane proton pump 58 kDa subunit,vacuolar proton pump 3,vacuolar proton pump, subunit 3
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