ATP5F1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ATP5F1 transfected lysate.
Western Blot
Lane 1: ATP5F1 transfected lysate ( 28.27 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP5F1 full-length
Host
Human
Theoretical MW (kDa)
28.27
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-KLRC1 antibody (H00000515-B01) by Western Blots.
SDS-PAGE Gel
ATP5F1 transfected lysate.
Western Blot
Lane 1: ATP5F1 transfected lysate ( 28.27 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP5F1
Entrez GeneID
515GeneBank Accession#
BC005366.1Protein Accession#
AAH05366.1Gene Name
ATP5F1
Gene Alias
MGC24431, PIG47
Gene Description
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit B1
Omim ID
603270Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq
Other Designations
ATP synthase B chain, mitochondrial|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit b, isoform 1|H+-ATP synthase subunit b|OTTHUMP00000013469|cell proliferation-inducing protein 47
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Interactome
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