ATP5B (Human) Recombinant Protein (P01)
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Specification
Product Description
Human ATP5B full-length ORF ( NP_001677.2, 1 a.a. - 529 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MLGFVGRVAAAPASGALRRLTPSASLPPAQLLLRAAPTAVHPVRDYAAQTSPSPKAGAATGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVLDSGAPIKIPVGPETLGRIMNVIGEPIDERGPIKTKQFAPIHAEAPEFMEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
83
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ATP5B
Entrez GeneID
506GeneBank Accession#
NM_001686.3Protein Accession#
NP_001677.2Gene Name
ATP5B
Gene Alias
ATPMB, ATPSB, MGC5231
Gene Description
ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Omim ID
102910Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core. [provided by RefSeq
Other Designations
ATP synthase, H+ transporting, mitochondrial F1 complex, beta subunit|mitochondrial ATP synthetase, beta subunit
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Interactome
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Publication Reference
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Diggin' on U(biquitin): A Novel Method for the Identification of Physiological E3 Ubiquitin Ligase Substrates.
Rubel CE, Schisler JC, Hamlett ED, Dekroon RM, Gautel M, Alzate O, Patterson C.
Cell Biochemistry and Biophysics 2013 Sep; 67(1):127.
Application：Func, Mouse, Heart.
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Enteroviruses and the pathogenesis of type 1 diabetes revisited: Cross-reactivity of enterovirus capsid protein (VP1) antibodies with human mitochondrial proteins.
Hansson SF, Korsgren S, Ponten F, Korsgren O.
Journal of Pathology 2013 Apr; 229(5):719.
Application：WB, Human, Recombinant protein.
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Diggin' on U(biquitin): A Novel Method for the Identification of Physiological E3 Ubiquitin Ligase Substrates.
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