ATP4B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ATP4B transfected lysate.
Western Blot
Lane 1: ATP4B transfected lysate ( 32.12 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP4B full-length
Host
Human
Theoretical MW (kDa)
32.12
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP4B antibody (H00000496-B02) by Western Blots.
SDS-PAGE Gel
ATP4B transfected lysate.
Western Blot
Lane 1: ATP4B transfected lysate ( 32.12 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP4B
Entrez GeneID
496GeneBank Accession#
NM_000705.2Protein Accession#
NP_000696.1Gene Name
ATP4B
Gene Alias
ATP6B
Gene Description
ATPase, H+/K+ exchanging, beta polypeptide
Omim ID
137217Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq
Other Designations
ATPase, H+/K+ transporting, beta polypeptide|gastric H+/K+ ATPase beta subunit|gastric hydrogen-potassium ATPase, beta|hydrogen/potassium-exchanging ATPase 4B|potassium-transporting ATPase beta chain|proton pump beta chain
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Interactome
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Disease
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