ATF3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ATF3 transfected lysate.
Western Blot
Lane 1: ATF3 transfected lysate ( 20.02 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATF3 full-length
Host
Human
Theoretical MW (kDa)
20.02
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATF3 antibody (H00000467-B01) by Western Blots.
SDS-PAGE Gel
ATF3 transfected lysate.
Western Blot
Lane 1: ATF3 transfected lysate ( 20.02 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATF3
Entrez GeneID
467GeneBank Accession#
NM_001674.2Protein Accession#
-Gene Name
ATF3
Gene Alias
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Gene Description
activating transcription factor 3
Omim ID
603148Gene Ontology
HyperlinkGene Summary
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [provided by RefSeq
Other Designations
ATF3deltaZip2|ATF3deltaZip2c|ATF3deltaZip3|OTTHUMP00000034887|OTTHUMP00000034890
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Interactome
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Disease
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