ASNS 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ASNS full-length
Host
Human
Theoretical MW (kDa)
61.82
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ASNS antibody (H00000440-B01) by Western Blots.
SDS-PAGE Gel
ASNS transfected lysate.
Western Blot
Lane 1: ASNS transfected lysate ( 61.82 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ASNS
Entrez GeneID
440GeneBank Accession#
NM_001673.2Protein Accession#
NP_001664.2Gene Name
ASNS
Gene Alias
TS11
Gene Description
asparagine synthetase
Omim ID
108370Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000024510|TS11 cell cycle control protein|glutamine-dependent asparagine synthetase
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Interactome
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Pathway
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