ASL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ASL transfected lysate.
Western Blot
Lane 1: ASL transfected lysate ( 51.15 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ASL full-length
Host
Human
Theoretical MW (kDa)
51.15
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ASL antibody (H00000435-B01) by Western Blots.
SDS-PAGE Gel
ASL transfected lysate.
Western Blot
Lane 1: ASL transfected lysate ( 51.15 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ASL
Entrez GeneID
435GeneBank Accession#
NM_000048.3Protein Accession#
NP_000039.2Gene Name
ASL
Gene Alias
ASAL
Gene Description
argininosuccinate lyase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000024494|OTTHUMP00000159903|argininosuccinase
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