ASAH1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ASAH1 transfected lysate.
Western Blot
Lane 1: ASAH1 transfected lysate ( 44.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ASAH1 full-length
Host
Human
Theoretical MW (kDa)
44.6
Interspecies Antigen Sequence
Mouse (79); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ASAH1 antibody (H00000427-D01P) by Western Blots.
SDS-PAGE Gel
ASAH1 transfected lysate.
Western Blot
Lane 1: ASAH1 transfected lysate ( 44.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ASAH1
Entrez GeneID
427GeneBank Accession#
NM_177924.1Protein Accession#
NP_808592.1Gene Name
ASAH1
Gene Alias
AC, ASAH, FLJ21558, FLJ22079, PHP, PHP32
Gene Description
N-acylsphingosine amidohydrolase (acid ceramidase) 1
Omim ID
228000Gene Ontology
HyperlinkGene Summary
This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
N-acylsphingosine amidohydrolase 1|OTTHUMP00000122482|acylsphingosine deacylase|putative 32 kDa heart protein
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Interactome
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Pathway
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Disease
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