ARRB2 monoclonal antibody (M01J), clone 3G1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ARRB2 expression in transfected 293T cell line by ARRB2 monoclonal antibody (M01J), clone 3G1.
Lane 1: ARRB2 transfected lysate (Predicted MW: 46.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARRB2 is 0.1 ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAPK3 and ARRB2. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-ARRB2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to ARRB2 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.84 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ARRB2.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
ARRB2 (AAH07427.1, 300 a.a. ~ 409 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NLASSTIVKEGANKEVLGILVSYRVKVKLVVSRGGDVSVELPFVLMHPKPHDHIPLPRPQSAAPETDVPVDTNLIEFDTNYATDDDIVFEDFARLRLKGMKDDDYDDQLC
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ARRB2 expression in transfected 293T cell line by ARRB2 monoclonal antibody (M01J), clone 3G1.
Lane 1: ARRB2 transfected lysate (Predicted MW: 46.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ARRB2 is 0.1 ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAPK3 and ARRB2. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-ARRB2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to ARRB2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — ARRB2
Entrez GeneID
409GeneBank Accession#
BC007427.2Protein Accession#
AAH07427.1Gene Name
ARRB2
Gene Alias
ARB2, ARR2, BARR2, DKFZp686L0365
Gene Description
arrestin, beta 2
Omim ID
107941Gene Ontology
HyperlinkGene Summary
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
arrestin 3
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Interactome
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Pathway
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Disease
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