RND3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RND3 transfected lysate.
Western Blot
Lane 1: RND3 transfected lysate ( 26.95 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RND3 full-length
Host
Human
Theoretical MW (kDa)
26.95
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RND3 antibody (H00000390-B02) by Western Blots.
SDS-PAGE Gel
RND3 transfected lysate.
Western Blot
Lane 1: RND3 transfected lysate ( 26.95 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RND3
Entrez GeneID
390GeneBank Accession#
NM_005168Protein Accession#
NP_005159Gene Name
RND3
Gene Alias
ARHE, Rho8, RhoE, memB
Gene Description
Rho family GTPase 3
Omim ID
602924Gene Ontology
HyperlinkGene Summary
Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM
Other Designations
OTTHUMP00000162685|ras homolog gene family, member E|small GTP binding protein Rho8
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