RHOA monoclonal antibody (M04J), clone 1B12
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant RHOA.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
RHOA (AAH01360.1, 1 a.a. ~ 193 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL
Host
Mouse
Reactivity
Human
Preparation Method
Cell Culture Production
Isotype
IgG1 Lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (46.97 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RHOA monoclonal antibody (M04J), clone 1B12. Western Blot analysis of RHOA expression in U-2 OS.Western Blot (Transfected lysate)
Western Blot analysis of RHOA expression in transfected 293T cell line by RHOA monoclonal antibody (M04J), clone 1B12.
Lane 1: RHOA transfected lysate (Predicted MW: 21.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to RHOA on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RHOA is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RHOA on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RHOA
Entrez GeneID
387GeneBank Accession#
BC001360.2Protein Accession#
AAH01360.1Gene Name
RHOA
Gene Alias
ARH12, ARHA, RHO12, RHOH12
Gene Description
ras homolog gene family, member A
Omim ID
165390Gene Ontology
HyperlinkGene Summary
O
Other Designations
Aplysia ras-related homolog 12|oncogene RHO H12|small GTP binding protein RhoA
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Interactome
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Disease
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