ARF4 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human ARF4 full-length ORF ( AAH03364, 1 a.a. - 180 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
45.54
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ARF4
Entrez GeneID
378GeneBank Accession#
BC003364Protein Accession#
AAH03364Gene Name
ARF4
Gene Alias
ARF2
Gene Description
ADP-ribosylation factor 4
Omim ID
601177Gene Ontology
HyperlinkGene Summary
This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor 2
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Interactome
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Publication Reference
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The A subunit of Escherichia coli heat-labile enterotoxin functions as a mucosal adjuvant and promotes IgG2a, IgA, and Th17 responses to vaccine antigens.
Norton EB, Lawson LB, Mahdi Z, Freytag LC, Clements JD.
Infection and Immunity 2012 Jul; 80(7):2426.
Application：Enzyme, Toxins and subunits.
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The A subunit of Escherichia coli heat-labile enterotoxin functions as a mucosal adjuvant and promotes IgG2a, IgA, and Th17 responses to vaccine antigens.
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