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Last updated: 2016/12/4

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APAF1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000317-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-APAF1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 137.1
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-APAF1 antibody (H00000317-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000317-T01
    APAF1 transfected lysate.
    Western Blot
    QC Testing of H00000317-T01
    Lane 1: APAF1 transfected lysate ( 137.1 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 317
  • Gene Name:
  • APAF1
  • Gene Alias:
  • CED4,DKFZp781B1145
  • Gene Description:
  • apoptotic peptidase activating factor 1
  • Gene Summary:
  • This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • apoptotic protease activating factor 1
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