AOC2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AOC2 transfected lysate.
Western Blot
Lane 1: AOC2 transfected lysate ( 83.27 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AOC2 full-length
Host
Human
Theoretical MW (kDa)
83.27
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AOC2 antibody (H00000314-B01) by Western Blots.
SDS-PAGE Gel
AOC2 transfected lysate.
Western Blot
Lane 1: AOC2 transfected lysate ( 83.27 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AOC2
Entrez GeneID
314GeneBank Accession#
NM_009590.2Protein Accession#
NP_033720.2Gene Name
AOC2
Gene Alias
DAO2, RAO
Gene Description
amine oxidase, copper containing 2 (retina-specific)
Omim ID
602268Gene Ontology
HyperlinkGene Summary
Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes and ammonia in the presence of copper and quinone cofactor. This gene shows high sequence similarity to copper amine oxidases from various species ranging from bacteria to mammals. The protein contains several conserved motifs including the active site of amine oxidases and the histidine residues that likely bind copper. It may be a critical modulator of signal transmission in retina, possibly by degrading the biogenic amines dopamine, histamine, and putrescine. This gene may be a candidate gene for hereditary ocular diseases. Alternate splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
amine oxidase, copper containing 2
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