ANXA3 monoclonal antibody (M12), clone 4F1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1.
Lane 1: ANXA3 transfected lysate(37.145 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANXA3 is approximately 1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of ANXA3 over-expressed 293 cell line, cotransfected with ANXA3 Validated Chimera RNAi ( Cat # H00000306-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA3 monoclonal antibody (M12), clone 4F1 (Cat # H00000306-M12 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (61.27 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ANXA3.
Immunogen
ANXA3 (AAH00871, 1 a.a. ~ 323 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIGTDEKMLISILTERSNAQRQLIVKEYQAAYGKELKDDLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKGAGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSLGDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLKLTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVNCVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRSEIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITLLKICGGDD
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (61.27 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1.
Lane 1: ANXA3 transfected lysate(37.145 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANXA3 is approximately 1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ANXA3 over-expressed 293 cell line, cotransfected with ANXA3 Validated Chimera RNAi ( Cat # H00000306-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA3 monoclonal antibody (M12), clone 4F1 (Cat # H00000306-M12 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — ANXA3
Entrez GeneID
306GeneBank Accession#
BC000871Protein Accession#
AAH00871Gene Name
ANXA3
Gene Alias
ANX3
Gene Description
annexin A3
Omim ID
106490Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq
Other Designations
Annexin III (lipocortin III)|annexin III (lipocortin III, 1,2-cyclic-inositol-phosphate phosphodiesterase, placental anticoagulant protein III, calcimedin 35-alpha)|calcimedin 35-alpha
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Interactome
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Publication Reference
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Noninvasive Precision Screening of Prostate Cancer by Urinary Multimarker Sensor and Artificial Intelligence Analysis.
Hojun Kim, Sungwook Park, In Gab Jeong, Sang Hoon Song, Youngdo Jeong, Choung-Soo Kim, Kwan Hyi Lee.
ACS Nano 2020 Dec; 15(3):4054.
Application：Func, Human, Human urine.
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Annexin A3 as a negative regulator of adipocyte differentiation.
Watanabe T, Ito Y, Sato A, Hosono T, Niimi S, Ariga T, Seki T.
Journal of Biochemistry 2012 Oct; 152(4):355.
Application：IF, IHC, WB, Mouse, 3T3-L1 cells, Mouse adipose tissue, Mouse mature adipocyte.
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A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer.
Cao DL, Ye DW, Zhang HL, Zhu Y, Wang YX, Yao XD.
Prostate 2011 May; 71(7):700.
Application：WB, Human, Human urine samples.
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Noninvasive Precision Screening of Prostate Cancer by Urinary Multimarker Sensor and Artificial Intelligence Analysis.
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