ANXA2 monoclonal antibody (M01), clone 3E8-B6
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More Files
- More Functions
Western Blot (Cell lysate)
ANXA2 monoclonal antibody (M01), clone 3E8-B6 Western Blot analysis of ANXA2 expression in Hela ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M01), clone 3E8-B6.
Lane 1: ANXA2 transfected lysate (Predicted MW: 40.4 KDa).
Lane 2: Non-transfected lysate.
RNAi Knockdown (Antibody validated)
Western blot analysis of ANXA2 over-expressed 293 cell line, cotransfected with ANXA2 Validated Chimera RNAi ( Cat # H00000302-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA2 monoclonal antibody (M01), clone 3E8-B6 (Cat # H00000302-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to ANXA2 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (63.03 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ANXA2.
Immunogen
ANXA2 (AAH09564.1, 1 a.a. ~ 339 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MFTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETLILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (63.03 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ANXA2 monoclonal antibody (M01), clone 3E8-B6 Western Blot analysis of ANXA2 expression in Hela ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ANXA2 expression in transfected 293T cell line by ANXA2 monoclonal antibody (M01), clone 3E8-B6.
Lane 1: ANXA2 transfected lysate (Predicted MW: 40.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ANXA2 over-expressed 293 cell line, cotransfected with ANXA2 Validated Chimera RNAi ( Cat # H00000302-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ANXA2 monoclonal antibody (M01), clone 3E8-B6 (Cat # H00000302-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to ANXA2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — ANXA2
Entrez GeneID
302GeneBank Accession#
BC009564Protein Accession#
AAH09564.1Gene Name
ANXA2
Gene Alias
ANX2, ANX2L4, CAL1H, LIP2, LPC2, LPC2D, P36, PAP-IV
Gene Description
annexin A2
Omim ID
151740Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000163650|annexin II|calpactin I heavy polypeptide|chromobindin 8|lipocortin II|placental anticoagulant protein IV
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Interactome
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Disease
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Publication Reference
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Annexin A2 is a discriminative serological candidate in early hepatocellular carcinoma.
Sun Y, Gao G, Cai J, Wang Y, Qu X, He L, Liu F, Zhang Y, Lin K, Ma S, Yang X, Qian X, Zhao X.
Carcinogenesis 2013 Mar; 34(3):595.
Application：S-ELISA, Recombinant protein.
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Peripheral blood monocyte-expressed ANXA2 geneis involved in pathogenesis of osteoporosis in humans.
Deng FY, Lei SF, Zhang Y, Zhang YL, Zheng YP, Zhang LS, Pan R, Wang L, Tian Q, Shen H, Zhao M, Lundberg YW, Liu YZ, Papasian CJ, Deng HW.
Molecular & Cellular proteomics: MCP 2011 Nov; 10(11):M111.01170.
Application：WB-Ce, Human, Peripheral blood monocytes.
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Annexin A2 is a discriminative serological candidate in early hepatocellular carcinoma.
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