ANXA1 monoclonal antibody (M10), clone 2D11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ANXA1.
Immunogen
ANXA1 (AAH01275, 1 a.a. ~ 346 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (89)
Isotype
IgG2a
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (63.8 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ANXA1 monoclonal antibody (M10), clone 2D11. Western Blot analysis of ANXA1 expression in human placenta.Western Blot (Cell lysate)
ANXA1 monoclonal antibody (M10), clone 2D11. Western Blot analysis of ANXA1 expression in HepG2(Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ANXA1 expression in transfected 293T cell line by ANXA1 monoclonal antibody (M10), clone 2D11.
Lane 1: ANXA1 transfected lysate(38.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANXA1 is 3 ng/ml as a capture antibody.ELISA
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Gene Info — ANXA1
Entrez GeneID
301GeneBank Accession#
BC001275Protein Accession#
AAH01275Gene Name
ANXA1
Gene Alias
ANX1, LPC1
Gene Description
annexin A1
Omim ID
151690Gene Ontology
HyperlinkGene Summary
Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq
Other Designations
OTTHUMP00000021474|OTTHUMP00000021475|annexin I|annexin I (lipocortin I)|lipocortin I
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Interactome
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Disease
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