AMY2A (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human AMY2A full-length ORF ( NP_000690.1, 1 a.a. - 511 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MKFFLLLFTIGFCWAQYSPNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVAIYNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVINHMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIENYNDATQVRDCRLTGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMWPGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFVPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTTCGNDWVCEHRWRQIRNMVIFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWSFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
84.1
Interspecies Antigen Sequence
Mouse (85); Rat (85)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — AMY2A
Entrez GeneID
279GeneBank Accession#
NM_000699.2Protein Accession#
NP_000690.1Gene Name
AMY2A
Gene Alias
AMY2, AMY2B, PA
Gene Description
amylase, alpha 2A (pancreatic)
Omim ID
104650Gene Ontology
HyperlinkGene Summary
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq
Other Designations
1,4-alpha-D-glucan glucanohydrolase|OTTHUMP00000012694|alpha-amylase|amylase, pancreatic, alpha-2A|found in the pancreas|glycogenase|pancreatic amylase 2A|pancreatic amylase alpha 2A
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Interactome
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Pathway
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Publication Reference
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Serological proteome analysis reveals new specific biases in the IgM and IgG autoantibody repertoires in autoimmune polyendocrine syndrome type 1.
Dubucquoi S, Proust-Lemoine E, Kemp EH, Ryndak A, Lefevre-Dutoit V, Bellart M, Saugier-Veber P, Duban-Deweer S, Wemeau JL, Prin L, Lefranc D.
Autoimmunity 2015 Aug; 48(8):532.
Application:WB, Human, Recombinant protein.
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Serological proteome analysis reveals new specific biases in the IgM and IgG autoantibody repertoires in autoimmune polyendocrine syndrome type 1.
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