AMT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AMT transfected lysate
Western Blot
Lane 1: AMT transfected lysate ( 31.9 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AMT full-length
Host
Human
Theoretical MW (kDa)
31.9
Interspecies Antigen Sequence
Mouse (89); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AMT antibody (H00000275-B01) by Western Blots.
SDS-PAGE Gel
AMT transfected lysate
Western Blot
Lane 1: AMT transfected lysate ( 31.9 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AMT
Entrez GeneID
275GeneBank Accession#
BC007546Protein Accession#
AAH07546Gene Name
AMT
Gene Alias
GCE, GCST, NKH
Gene Description
aminomethyltransferase
Gene Ontology
HyperlinkGene Summary
The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM
Other Designations
glycine cleavage system protein T
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Interactome
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Pathway
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Disease
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