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AMT 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000275-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-AMT full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 31.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-AMT antibody (H00000275-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000275-T01
    AMT transfected lysate
    Western Blot
    QC Testing of H00000275-T01
    Lane 1: AMT transfected lysate ( 31.9 KDa).
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 275
  • Gene Name:
  • AMT
  • Gene Alias:
  • GCE,GCST,NKH
  • Gene Description:
  • aminomethyltransferase
  • Gene Summary:
  • The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM
  • Other Designations:
  • glycine cleavage system protein T
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