AMPD2 monoclonal antibody (M04A), clone 2G8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant AMPD2.
Immunogen
AMPD2 (NP_631895, 86 a.a. ~ 185 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ISQDVKLEPDILLRAKQDFLKTDSDSDLQLYKEQGEGQGDRSLRERDVLEREFQRVTISGEEKCGVPFTDLLDAAKSVVRALFIREKYMALSLQSFCPTT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In ascites fluid
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of AMPD2 expression in transfected 293T cell line by AMPD2 monoclonal antibody (M04A), clone 2G8.
Lane 1: AMPD2 transfected lysate(92.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — AMPD2
Entrez GeneID
271GeneBank Accession#
NM_139156Protein Accession#
NP_631895Gene Name
AMPD2
Gene Alias
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Gene Description
adenosine monophosphate deaminase 2 (isoform L)
Omim ID
102771Gene Ontology
HyperlinkGene Summary
Adenosine monophosphate deaminase-2 (EC 3.5.4.6) catalyzes the deamination of AMP to IMP and plays an important role in the purine nucleotide cycle.[supplied by OMIM
Other Designations
AMP deaminase 2|OTTHUMP00000013369|OTTHUMP00000013371
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Interactome
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Pathway
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Disease
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Publication Reference
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Effects of Pharmacological AMP Deaminase Inhibition and Ampd1 Deletion on Nucleotide Levels and AMPK Activation in Contracting Skeletal Muscle.
Plaideau C, Lai YC, Kviklyte S, Zanou N, Lofgren L, Andersen H, Vertommen D, Gailly P, Hue L, Bohlooly-Y M, Hallen S, Rider MH.
Chemistry & Biology 2014 Nov; 21(11):1497.
Application:WB-Ti, Mouse, Skeletal muscles.
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Uric acid-dependent inhibition of AMP kinase induces hepatic glucose production in diabetes and starvation: evolutionary implications of the uricase loss in hominids.
Cicerchi C, Li N, Kratzer J, Garcia G, Roncal-Jimenez CA, Tanabe K, Hunter B, Rivard CJ, Sautin YY, Gaucher EA, Johnson RJ, Lanaspa MA.
FASEB Journal 2014 Aug; 28(8):3339.
Application:WB-Ti, WB-Tr, Mouse, Liver, HepG2 cells.
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Effects of Pharmacological AMP Deaminase Inhibition and Ampd1 Deletion on Nucleotide Levels and AMPK Activation in Contracting Skeletal Muscle.
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