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Last updated: 2017/4/23
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ALPL 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000249-T01
  • Visit Frequency :
  • Countries :
  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ALPL full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 57.75
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALPL antibody (H00000249-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000249-T01
    ALPL transfected lysate.
    Western Blot
    QC Testing of H00000249-T01
    Lane 1: ALPL transfected lysate ( 57.75 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 249
  • Protein Accession#:
  • -
  • Gene Name:
  • ALPL
  • Gene Alias:
  • AP-TNAP,FLJ40094,FLJ93059,HOPS,MGC161443,MGC167935,TNAP,TNSALP
  • Gene Description:
  • alkaline phosphatase, liver/bone/kidney
  • Gene Summary:
  • There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000002971,OTTHUMP00000002972,alkaline phosphatase, tissue-nonspecific isozyme,alkaline phosphomonoesterase,glycerophosphatase,liver/bone/kidney-type alkaline phosphatase,tissue non-specific alkaline phosphatase,tissue-nonspecific ALP
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