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ALDOB 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000229-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-ALDOB full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 39.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ALDOB antibody (H00000229-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000229-T01
    ALDOB transfected lysate.
    Western Blot
    QC Testing of H00000229-T01
    Lane 1: ALDOB transfected lysate ( 39.5 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 229
  • Gene Name:
  • ALDOB
  • Gene Alias:
  • -
  • Gene Description:
  • aldolase B, fructose-bisphosphate
  • Gene Summary:
  • Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000021803,aldolase 2,aldolase B, fructose-bisphosphatase
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