ALDH2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALDH2 full-length
Host
Human
Theoretical MW (kDa)
56.4
Interspecies Antigen Sequence
Mouse (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALDH2 antibody (H00000217-D01P) by Western Blots.
SDS-PAGE Gel
ALDH2 transfected lysate.
Western Blot
Lane 1: ALDH2 transfected lysate ( 56.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ALDH2
Entrez GeneID
217GeneBank Accession#
NM_000690.2Protein Accession#
NP_000681.2Gene Name
ALDH2
Gene Alias
ALDH-E2, ALDHI, ALDM, MGC1806
Gene Description
aldehyde dehydrogenase 2 family (mitochondrial)
Omim ID
100650Gene Ontology
HyperlinkGene Summary
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq
Other Designations
ALDH class 2|acetaldehyde dehydrogenase 2|liver mitochondrial ALDH|mitochondrial aldehyde dehydrogenase 2|nucleus-encoded mitochondrial aldehyde dehydrogenase 2
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Interactome
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Disease
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