This antibody pair set comes with matched antibody pair to detect and quantify protein level of human AKT1.
Quality Control Testing:
Standard curve using recombinant protein ( H00000207-P01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 0.1 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-AKT1, IgG2a Kappa (100 ug) 2. Detection antibody: biotinylated mouse monoclonal anti-AKT1, IgG2a Lambda (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq