AK1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
AK1 transfected lysate.
Western Blot
Lane 1: AK1 transfected lysate ( 21.45 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AK1 full-length
Host
Human
Theoretical MW (kDa)
21.45
Interspecies Antigen Sequence
Mouse (89); Rat (90)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AK1 antibody (H00000203-B01) by Western Blots.
SDS-PAGE Gel
AK1 transfected lysate.
Western Blot
Lane 1: AK1 transfected lysate ( 21.45 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AK1
Entrez GeneID
203GeneBank Accession#
NM_000476.1Protein Accession#
-Gene Name
AK1
Gene Alias
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Gene Description
adenylate kinase 1
Omim ID
103000Gene Ontology
HyperlinkGene Summary
Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme. [provided by RefSeq
Other Designations
ATP-AMP transphosphorylase|OTTHUMP00000022217|OTTHUMP00000022218|myokinase
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Interactome
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Pathway
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Disease
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