AGL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AGL transfected lysate.
Western Blot
Lane 1: AGL transfected lysate ( 168.63 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AGL full-length
Host
Human
Theoretical MW (kDa)
168.63
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AGL antibody (H00000178-B01) by Western Blots.
SDS-PAGE Gel
AGL transfected lysate.
Western Blot
Lane 1: AGL transfected lysate ( 168.63 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AGL
Entrez GeneID
178GeneBank Accession#
BC078663.1Protein Accession#
NP_000019.2Gene Name
AGL
Gene Alias
GDE
Gene Description
amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Ontology
HyperlinkGene Summary
This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000012500|OTTHUMP00000012501|OTTHUMP00000012502|OTTHUMP00000012503|OTTHUMP00000012504|OTTHUMP00000012505|glycogen debranching enzyme
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Interactome
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Pathway
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Disease
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