AFP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AFP transfected lysate.
Western Blot
Lane 1: AFP transfected lysate ( 67.1 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AFP full-length
Host
Human
Theoretical MW (kDa)
67.1
Interspecies Antigen Sequence
Mouse (65); Rat (65)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AFP antibody (H00000174-B01) by Western Blots.
SDS-PAGE Gel
AFP transfected lysate.
Western Blot
Lane 1: AFP transfected lysate ( 67.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AFP
Entrez GeneID
174GeneBank Accession#
NM_001134Protein Accession#
NP_001125Gene Name
AFP
Gene Alias
FETA, HPAFP
Gene Description
alpha-fetoprotein
Omim ID
104150Gene Ontology
HyperlinkGene Summary
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq
Other Designations
OTTHUMP00000160480|alpha-1-fetoprotein|alpha-fetoglobulin
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Interactome
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