CRISP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CRISP1 transfected lysate.
Western Blot
Lane 1: CRISP1 transfected lysate ( 28.5 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CRISP1 full-length
Host
Human
Theoretical MW (kDa)
28.5
Interspecies Antigen Sequence
Rat (63)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CRISP1 antibody (H00000167-B01) by Western Blots.
SDS-PAGE Gel
CRISP1 transfected lysate.
Western Blot
Lane 1: CRISP1 transfected lysate ( 28.5 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CRISP1
Entrez GeneID
167GeneBank Accession#
NM_001131Protein Accession#
NP_001122Gene Name
CRISP1
Gene Alias
AEGL1, ARP, CRISP-1, HSCRISP1D, HSCRISP1G, HUMARP
Gene Description
cysteine-rich secretory protein 1
Omim ID
601193Gene Ontology
HyperlinkGene Summary
Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq
Other Designations
AEG-related protein|OTTHUMP00000016592|acidic epididymal glycoprotein-like 1|cysteine-rich secretory protein-1 delta|protein DE-like
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