ACYP2 monoclonal antibody (M06), clone 2H7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ACYP2 expression in transfected 293T cell line by ACYP2 monoclonal antibody (M06), clone 2H7.
Lane 1: ACYP2 transfected lysate(11.1 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACYP2 is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of ACYP2 over-expressed 293 cell line, cotransfected with ACYP2 Validated Chimera RNAi ( Cat # H00000098-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ACYP2 monoclonal antibody (M06), clone 2H7 (Cat # H00000098-M06 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.52 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ACYP2.
Immunogen
ACYP2 (NP_612457, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTAQSLKSVDYEVFGRVQGVCFRMYTEDEARKIGVVGWVKNTSKGTVTGQVQGPEDKVNSMKSWLSKVGSPSSRIDRTNFSNEKTISKLEYSNFSIR
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (84); Rat (88)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ACYP2 expression in transfected 293T cell line by ACYP2 monoclonal antibody (M06), clone 2H7.
Lane 1: ACYP2 transfected lysate(11.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ACYP2 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of ACYP2 over-expressed 293 cell line, cotransfected with ACYP2 Validated Chimera RNAi ( Cat # H00000098-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ACYP2 monoclonal antibody (M06), clone 2H7 (Cat # H00000098-M06 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — ACYP2
Entrez GeneID
98GeneBank Accession#
NM_138448Protein Accession#
NP_612457Gene Name
ACYP2
Gene Alias
ACYM, ACYP
Gene Description
acylphosphatase 2, muscle type
Omim ID
102595Gene Ontology
HyperlinkGene Summary
Acylphosphatase can hydrolyze the phosphoenzyme intermediate of different membrane pumps, particularly the Ca2+/Mg2+-ATPase from sarcoplasmic reticulum of skeletal muscle. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase on the basis of their tissue localization. This gene encodes the muscle-type isoform (MT). An increase of the MT isoform is associated with muscle differentiation. [provided by RefSeq
Other Designations
acylphosphate phosphohydrolase|muscle-type acylphosphatase 2
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Interactome
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Pathway
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Disease
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