This antibody pair set comes with matched antibody pair to detect and quantify protein level of human ACTA2.
Quality Control Testing:
Standard curve using recombinant protein ( H00000059-P01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-ACTA2, IgG2b Kappa (100 ug) 2. Detection antibody: biotinylated mouse monoclonal anti-ACTA2, IgG1 Kappa (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Sandwich ELISA detection sensitivity ranging from approximately 6561x to 729x dilution of the ACTA2 293T overexpression lysate (non-denatured). Standard curve using ACTA2 293T overexpression lysate (non-denatured) as an analyte.
The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq