ACRV1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ACRV1 transfected lysate.
Western Blot
Lane 1: ACRV1 transfected lysate ( 28.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACRV1 full-length
Host
Human
Theoretical MW (kDa)
28.2
Interspecies Antigen Sequence
Mouse (62); Rat (61)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACRV1 antibody (H00000056-B02) by Western Blots.
SDS-PAGE Gel
ACRV1 transfected lysate.
Western Blot
Lane 1: ACRV1 transfected lysate ( 28.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ACRV1
Entrez GeneID
56GeneBank Accession#
NM_001612Protein Accession#
NP_001603Gene Name
ACRV1
Gene Alias
D11S4365, SP-10, SPACA2
Gene Description
acrosomal vesicle protein 1
Omim ID
102525Gene Ontology
HyperlinkGene Summary
This gene encodes a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. This gene consists of 4 exons and its alternative splicing generates multiple distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for < 1% of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. [provided by RefSeq
Other Designations
sperm protein 10
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