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Last updated: 2016/10/23
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AARS 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00000016-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-AARS full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 106.59
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-AARS antibody (H00000016-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00000016-T01
    AARS transfected lysate.
    Western Blot
    QC Testing of H00000016-T01
    Lane 1: AARS transfected lysate ( 106.59 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 16
  • Gene Name:
  • AARS
  • Gene Alias:
  • -
  • Gene Description:
  • alanyl-tRNA synthetase
  • Gene Summary:
  • The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq
  • Other Designations:
  • alanine tRNA ligase 1, cytoplasmic
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