Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique.
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Representative images of human glioma tissue stain with the FISH probe. The left image is two signals of MDM2 gene (Orange) in a normal cell; the right image is higher copy number of MDM2 gene (amplification) without chromosome enumeration probe.
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Hybridization position of the probes on the chromosome.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq