Labeled FISH probes for identification of gene amplification using Fluoresecent In Situ Hybridization Technique.
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Representative images of human cell stain with the dual color FISH probe. The left image is two copies of CCND1 gene in HL60S cell line; and the right image is higher copy number of CCND1 gene (amplification) without chromosome enumeration probe in SKMEL2 cell line.
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Hybridization position of the probes on the chromosome.
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. [provided by RefSeq